Fecha/hora: 01/06/2024 10:20:10

Biblioteca(s): 	INIA La Estanzuela; INIA Las Brujas.	
Fecha corriente: 	22/07/2018	
Fecha de la última actualización: 	09/10/2019	
Autoria: 	SILVEIRA, C.S.; CESAR, D.; KEATING, M.K.; DELEON-CARNES, M.; ARMIÉN, A.; LUHERS, M.; RIET-CORREA, F.; GIANNITTI, F.	
Afiliación: 	CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DEBORAH CESAR, Private Practice, Uruguay; M. KELLY KEATING, Centers for Disease Control and Prevention, Atlanta, GA, USA; MARLENE DELEON-CARNES, Centers for Disease Control and Prevention, Atlanta, GA, USA; ANÍBAL ARMIÉN, Universidad de Minnesota; MARTÍN LUHERS, Universidad de la República (UdelaR); FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.	
Título: 	A case of prototheca zopfii genotype 1 infection in a dog (Canis lupus familiaris).	
Complemento del título: 	Case Report.	
Año de publicación: 	2018	
Fuente/Imprenta: 	Mycopathologia, June 2018. [First Online]	
ISSN: 	0301-486X / Online: 1573-0832	
DOI: 	10.1007/s11046-018-0274-5	
Idioma: 	Inglés	
Notas: 	Article history: First Online: 06 June 2018.	
Contenido: 	ABSTRACT. 
Protothecosis is a rare disease caused by environmental algae of the genus Prototheca. These are saprophytic, non-photosynthetic, aerobic, colorless algae that belong to the Chlorellaceae family. Seven different species have been described. Prototheca zopfii genotype 2 and P. wickerhamii are most commonly involved in pathogenic infections in humans and animals. The objective of this work is to describe, for the first time, a case of protothecosis caused by P. zopfii genotype 1 in a dog. The dog, a 4-year-old mix bred male, was presented to a veterinary clinic in Montevideo, Uruguay, with multiple skin nodules, one of which was excised by surgical biopsy. The sample was examined histologically and processed by PCR, DNA sequencing, and restriction fragments length polymorphisms for the detection and genotyping of P. zopfii. In addition, transmission electron microscopy and scanning electron microscopy were performed. Histology showed severe ulcerative granulomatous dermatitis and panniculitis with myriads of pleomorphic algae. Algal cells were 4?17 µm in size, with an amphophilic, 2?4-µm-thick wall frequently surrounded by a clear halo, contained flocculant material and a deeply basophilic nucleus, and internal septae with daughter cells (endospores) consistent with endosporulation. Ultrastructurally, algal cells/endospores at different stages of development were found within parasitophorous vacuoles in macrophages. Prototheca zopfii genotype 1 was identified by molecular testing, confirming the etiologic diagnosis of protothecosis. © Springer	
Palabras-Clave: 	Algal diseases; Algal infection; Dog; ENFERMEDADES CAUSADAS POR ALGAS; Genotypic characterization; PLATAFORMA SALUD ANIMAL; PROTOTECOSIS; Protothecosis; SOUTH AMERICA.	
Thesagro: 	ENFERMEDADES INFECCIOSAS; PERRO; SUD AMERICA.	
Categoría del tema: 	--
L73 Enfermedades de los animales	
Registro original: 	INIA Las Brujas (LB)	
Ejemplares: 	LB - (101651-1) - PXI - AP - PP - PP/MYCOPATHOLOGIA/2018 -  -  - DIS
LE - (102510-1) - PXI - AP - DD -  -  -  - DIS